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KMID : 0903519610020010036
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1961 Volume.2 No. 1 p.36 ~ p.39
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Isolation of Metabolites of S35(1,2 - dichloro vinyl) L - Cysteine in Bovine
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Abstract
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It has been ascertained that S-(1, 2-dichlorovinyl) L-cysteine (DCVC) is probably the toxic component in the trichloro-ethylene extracted soybean oil meal on the bovine.
For the study of the metabolites of DCVC, the components with radioactive S^(35)-in the urine of the calf, to which S^(35)-DCVC was administrated, were separated using of cellulose powder with propanol-water (70:30 V/V) which is easily removable by evaporation.
As the results followings were obtained:
Peak 1, which shows fractions containing single S^(35)-radioactive components, whose Rf is 0.815
Peak 2, which shows fractions containing jingle S^(35)-radioactive component, whose Rf is 0.447
Peak 3, which shows fractions containing both S^(35)-radioactive components whose Rfs are 0.090 and 0.171
Peak 4, which shows fractions containing single S^(35)-radioactive component, whose Rf is 0.142.
Peak 5, which shows fractions containing single S^(35)-radioactive component, whose Rf is 0.084.
Besides these, two of small peak were obtained.
Although, the resolving power of the cellulose powder column is not sufficient, it is applicable for the study of the components of metabolytes of DCVC conveniently with the rest of removable solvent easily.
Also the components with radioactive S^(35) in the feces of the calf were separated using citrate buffer gradient system of Moore and stein.
As the results;
three S^(35)-radioactive components were separated.
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